Use of a reverse dot blot procedure to identify the presence of multiple serovars in Chlamydia trachomatis urogenital infection.
نویسنده
چکیده
Epidemiologic research requires identification of Chlamydia trachomatis serovars and detection of mixed infection. Antibody-based serotyping is unworkable when specimens are urine or vaginal swabs. We developed a reverse dot blot (RDB) to screen for multiple serotypes in these specimens. RDB yielded the predicted results on all artificially mixed samples and on seven of eight clinically mixed samples.
منابع مشابه
Combination of PCR targeting the VD2 of omp1 and reverse line blot analysis for typing of urogenital Chlamydia trachomatis serovars in cervical scrape specimens.
In this study we developed and evaluated a new PCR-based typing assay, directed to the VD2 region of the omp1 gene, for the detection and typing of urogenital Chlamydia trachomatis infections. A nested VD2 PCR-reverse line blot (RLB) assay was developed for the typing of nine different urogenital serovars of C. trachomatis. The assay developed was tested with reference strains of C. trachomatis...
متن کاملSerovar distribution of urogenital Chlamydia trachomatis strains
The distribution of serovars in 208 Chlamydia trachomatis strains of urogenital origin isolated from 185 patients attending a sexually transmitted disease clinic in Rotterdam, The Netherlands, was assessed. Typing by monoclonal antisera using a dot enzyme linked immunosorbent assay (ELISA) showed that the most common serovars were E (found in 45 strains), F (39), D (34), and K (28). Other serov...
متن کاملThe Prevalence of Chlamydia trachomatis Infection in Women Attending Health Clinics in Shiraz, Islamic Republic of Iran
Background Chlamydia trachomatis (CT) is a common cause of sexually transmitted infections (STI) and a prevalent microorganism found in the vaginal discharge of sexually active women. The infection usually has no symptoms; although it may cause chronic complications such as pelvic inflammatory disease (PID), ectopic pregnancy, chronic pelvic pain, tubal infertility and cervical cancer. This stu...
متن کاملUsing Recombinant Chlamydia Major Outer Membrane Protein (MOMP) in ELISA Diagnostic Kit
Chlamydia trachomatis is one of the main causes of Sexually Transmitted Diseases (STDs) such as prostatitis and epididymitis in men and cervicitis, endometriosis, vaginitis and ureogenital tract infections in women. Serological tests with sensitivities related to specific antigens are commonly used as routine laboratory tests for diagnosis of Chlamydia. In this research the Chlamydia Major O...
متن کاملEvaluation of serotyping using monoclonal antibodies and PCR-RFLP for Chlamydia trachomatis serotype identification.
We compared genotyping by restriction fragment length polymorphism (RFLP) analysis of the amplified omp1 gene with serotyping by dot enzyme-linked immunosorbent assay (dot-ELISA) to determine the suitability of RFLP analysis for epidemiologic study. Fifteen prototypes of Chlamydia trachomatis and 30 clinical isolates were used in this study. To serotype with dot-ELISA, chlamydia antigen was spo...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of clinical microbiology
دوره 39 7 شماره
صفحات -
تاریخ انتشار 2001